A Quantitative Method for Fungal Ligninolytic Enzyme Screening Studies

Asia-Pacific Journal of Chemical Engineering, 6, 589-595.

18 Pages Posted: 19 Jan 2015

See all articles by Teck Nam

Teck Nam

The University of Auckland

Gek-Cheng Ngoh

University of Malaya (UM) - Department of Chemistry

Adeline Seak May Chua

University of Malaya (UM)

Date Written: 2011

Abstract

Screening is conducted to select a fungus with desired characteristics intended for various applications, e.g. bioremediation and enzyme production. A qualitative method was used in this study for screening of fungal ligninolytic enzymes activities. The fungal ligninolytic activity was correlated with its growth from the screenings to identify a suitable fungus for solid substrate fermentation. Four strains of fungi, namely Phanerochaete chrysosporium, Pycnoporus sanguineus, Phlebia radiata and Pleurotus sajor-caju, were screened for their ligninolytic enzymes activities using guaiacol and Remazol Brilliant Blue-R (RBB-R) as screening reagents. The screenings were conducted at both the optimal growth temperature of each fungus and 35oC. All the fungi, except P. radiata, showed positive guaiacol oxidation and RBB-R decolourization activities. Based on the results, P. sajor-caju may be a suitable fungus for use in bioconversion of lignocellulose because it maintained guaiacol oxidation and RBB-R decolourization activities at 35oC. Its halo to colony areas ratios of guaiacol oxidation and RBB-R decolourization after 6 days were 7.0 and 2.7, respectively. In the subsequent growth study, the measured colony area was closely correlated with the dry fungal cell weight with a regression value (R2) of 0.971. Thus, colony size could be used as an indicator for fungal growth if the screening media do not significantly slow the growth of fungus.

Keywords: Quantitative method; Ligninolytic enzymes; Screening; Guaiacol oxidation; Remazol Brilliant Blue-R (RBB-R) decolourization; Lignocellulose.

JEL Classification: C09, C93, N55, O13, Q00, Q10, Q16, Q20, Q23, Q40, Q42

Suggested Citation

Ang, Teck Nam and Ngoh, Gek-Cheng and Chua, Adeline Seak May, A Quantitative Method for Fungal Ligninolytic Enzyme Screening Studies (2011). Asia-Pacific Journal of Chemical Engineering, 6, 589-595., Available at SSRN: https://ssrn.com/abstract=2550026

Teck Nam Ang (Contact Author)

The University of Auckland ( email )

Department of Chemical and Materials Engineering
Auckland, AK 1010
New Zealand

Gek-Cheng Ngoh

University of Malaya (UM) - Department of Chemistry

Jalan Lembah Pantai
Kuala Lumpur, Wilayah Persekutuan 50603
Malaysia

Adeline Seak May Chua

University of Malaya (UM) ( email )

Kuala Lumpur, Wilayah Persekutuan 50603
University of Malaya (UM)
Kuala Lumpur, Wilayah Persekutuan 50603
Malaysia

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