A Quantitative Method for Fungal Ligninolytic Enzyme Screening Studies
Asia-Pacific Journal of Chemical Engineering, 6, 589-595.
18 Pages Posted: 19 Jan 2015
Date Written: 2011
Screening is conducted to select a fungus with desired characteristics intended for various applications, e.g. bioremediation and enzyme production. A qualitative method was used in this study for screening of fungal ligninolytic enzymes activities. The fungal ligninolytic activity was correlated with its growth from the screenings to identify a suitable fungus for solid substrate fermentation. Four strains of fungi, namely Phanerochaete chrysosporium, Pycnoporus sanguineus, Phlebia radiata and Pleurotus sajor-caju, were screened for their ligninolytic enzymes activities using guaiacol and Remazol Brilliant Blue-R (RBB-R) as screening reagents. The screenings were conducted at both the optimal growth temperature of each fungus and 35oC. All the fungi, except P. radiata, showed positive guaiacol oxidation and RBB-R decolourization activities. Based on the results, P. sajor-caju may be a suitable fungus for use in bioconversion of lignocellulose because it maintained guaiacol oxidation and RBB-R decolourization activities at 35oC. Its halo to colony areas ratios of guaiacol oxidation and RBB-R decolourization after 6 days were 7.0 and 2.7, respectively. In the subsequent growth study, the measured colony area was closely correlated with the dry fungal cell weight with a regression value (R2) of 0.971. Thus, colony size could be used as an indicator for fungal growth if the screening media do not significantly slow the growth of fungus.
Keywords: Quantitative method; Ligninolytic enzymes; Screening; Guaiacol oxidation; Remazol Brilliant Blue-R (RBB-R) decolourization; Lignocellulose.
JEL Classification: C09, C93, N55, O13, Q00, Q10, Q16, Q20, Q23, Q40, Q42
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